Research tools
Peptide calculator
Work out the concentration of a reconstituted research solution. Enter the amount of lyophilised material in the vial and the volume of diluent added, and the calculator returns the resulting concentration in milligrams per millilitre.
Enter the two values
This tool performs arithmetic only. It does not provide a protocol, a recommended amount, or instructions for human or animal use.
What the calculation does
Research peptides are supplied as a lyophilised (freeze-dried) powder, because peptides in solution degrade far faster than peptides kept dry. Before any analytical work, the powder is reconstituted: a measured volume of diluent is added to the vial and the solid dissolves into it.
The arithmetic is a single division. Concentration equals the mass in the vial divided by the volume of diluent added. A 10mg vial reconstituted in 2mL gives 5 mg/mL; the same vial in 1mL gives 10 mg/mL. Nothing about the peptide changes, only how much liquid it is dissolved in.
Why the vial amount is not always the peptide amount
For most single compounds the labelled milligram figure and the peptide content line up. For salts and co-ordinated complexes they do not. GHK-Cu is the clearest example: it is a tripeptide in complex with copper, so a vial labelled 50mg contains more than 50mg of total material once the copper is counted. The released batch report states the split explicitly rather than leaving it implied.
Peptide blends have the same issue in a different form. A blend labelled with one combined weight is really several peptides in fixed proportion, and the concentration of any one component depends on the composition table in the batch record, not on the headline number.
Check the batch record, not the label
A calculation is only as good as the number it starts from. The mass a laboratory actually measured in a vial is reported on the batch-specific certificate of analysis, and it commonly differs slightly from the labelled pack size. Every Pillar Research batch publishes that figure alongside the identity and purity results.
The COA review checklist covers what a complete report should contain, and the identity-versus-purity guide explains why those are two separate analytical questions.
Research use only
This calculator is provided for in vitro laboratory and educational research. It describes the concentration of a prepared solution and nothing else. It is not a protocol, does not recommend an amount, and does not provide instructions for human or animal use. Compounds referenced on this site are not listed on the Australian Register of Therapeutic Goods (ARTG) and are not therapeutic goods under the Therapeutic Goods Act 1989 (Cth).